Journal: Cell Death & Disease

Article Title: SNP-mediated lncRNA-ENTPD3-AS1 upregulation suppresses renal cell carcinoma via miR-155/HIF-1α signaling

doi: 10.1038/s41419-021-03958-4

Figure Lengend Snippet: A The genomic location of rs67311347 and ENTPD3-AS1. B Epigenetic tracks obtained from ENCODE database showed the enrichment of enhancer marks (H3K27ac peaks, H3K4me1 peaks) and transcription marks (H3K4me3 peaks) in the rs67311347 region. C The Polymerase II ChIP-PCR showed that Polymerase II occupied the rs67311347 region. D Relative reporter gene activity of the constructs containing the WT or Mut allele of rs67311347 in forward orientation in A498 cells. E The altered allele A of rs67311347 created a ZNF8 binding motif. F ChIP-qPCR of ZNF8 in RCC cell lines carrying different genotypes of rs67311347.

Article Snippet: The DNA–protein complexes were pre-cleared with Protein A Agarose/Salmon DNA and then immunoprecipitated with anti-ZNF8 antibody (Novus Biologicals, USA), anti-Pol II antibody (Novus Biologicals, USA), and normal rabbit/mouse IgG.

Techniques: Activity Assay, Construct, Binding Assay, ChIP-qPCR

Journal: Inflammation

Article Title: L-selectin Promotes Migration, Invasion and Inflammatory Response of Fibroblast-Like Synoviocytes in Rheumatoid Arthritis via NF-kB Signaling Pathway.

doi: 10.1007/s10753-025-02242-3

Figure Lengend Snippet: Fig.4 Knockdown of L-selectin reduced arthritis severity in CIA rats through the NF-κB signaling pathway. (A) The image of the rats' hind paws. (B) The paw thickness and arthritis index for different groups. (C) Representative images of H&E staining of ankle joint sections. Scale bar, 200 µM. (D) Representative images of Safranin O/Fast Green staining of ankle joint sections. Scale bar, 200 µM. (E) TNF-α, IL-1β, and IL-6 levels in the serum of the different groups were analyzed by ELISA. (F) The protein expression levels of L-selectin, p65, p-p65, IκBα, and p-IκBα were assessed via western blot. *p < 0.05 compared with CIA + siCtrl group. #p < 0.05 compared with CIA + si-L-selectin group. Data are presented as the mean ± SD of at least three independent experiments

Article Snippet: The membrane was blocked with 5% skim milk on a shaker for 2 h at room temperature, then incubated overnight at 4 °C with a primary antibody as follows: L-selectin (58225S, Cell Signaling, USA), GAPDH (60,004–1-Ig, Proteintech, China), p65 (10,745–1-AP, Proteintech, China), p-p65 (82,335–1-RR, Proteintech, China), IκBα (10,268–1-AP, Proteintech, China) and p-IκBα (82,349–1-RR, Proteintech, China).

Techniques: Knockdown, Staining, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot

Journal: Frontiers in Immunology

Article Title: PARP2 downregulation in T cells ameliorates lipopolysaccharide-induced inflammation of the large intestine

doi: 10.3389/fimmu.2023.1135410

Figure Lengend Snippet: Regulatory T cells in the large intestinal mucosa. (A) Number of FoxP3 positive cells in the cecum per mm 2 . The number of regulatory T cells was increased after LPS treatment only in the T-cell-PARP2-KO group. (B) Representative images of FoxP3 labeled colonic sections. Brown-colored diaminobenzidine represents specific staining, the counterstaining is blue-colored hematoxylin. Blue arrows show positively stained cells. The scale bar is 50µm. Lines represent mean ± SD, Two-way ANOVA, *: p<0.05, **p<0.001; N=3-4 animals/group.

Article Snippet: The intestinal localization of regulatory T cells was examined by the immunohistochemical labeling of FoxP3 (pH3 citrate buffer antigen retrieval for 17 min, FoxP3 (14-5773-82) rat monoclonal IgG2a kappa antibody 1:500, Invitrogen, Massachusetts, USA; RRID: AB_467576 and isotype control (14-4321-82) rat IgG2a kappa antibody 1:500, Invitrogen, Massachusetts, USA; RRID: AB_470105).

Techniques: Labeling, Staining